Molecular Characterisation of the GdhA- Derivative of Pasteurella multocida B:2

Pasteurella multocida B:2 is an important veterinary pathogen causing fatal and acute haemorrhagic septicaemia (HS) in bovine. A live vaccine candidate, P. GDH7 was reported to enable protection cattle buffaloes via intranasal (i. n.) administration. This potential also be self-transmitted from the vaccinated animal free-ranging animals allowing wider vaccination coverage. Prior commercialisation, this requires further characterisation accordance with authoritative guidelines World Organisation for Animal Health (OIE). Hence, study, strain, virulent parent strain were characterised through genomic proteomic profiling. crucial first step develop a sensitive yet simple robust identification test differentiate both strains which has been achieved by development of precise straightforward PCR method. In profiling, Repetitive Extragenic Palindromic sequence-PCR (REP-PCR) manipulated have different display DNA band patterns. Some major OMPs observed prominent immunogens multocida, OmpA OmpH expressed differently between these SDS-PAGE analysis. conclusion, reproducible detection method enabled differentiation strains. Further shows significantly profile